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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
Cut at G^GNCC sites with Cfr13I (Sau96I) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AspS9I, BmgT120I, PspPI, Sau96I).
Cut at CTGGAG(16/14)^ sites with Thermo Scientific™ FastDigest™ BpmI (GsuI), which performs best at 30°C in 5-15 minutes using universal FastDigest Buffer.
Cut at RG^GNCCY sites with Thermo Scientific™ FastDigest™ EcoO109I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: DraII).
Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
Routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means.